PA5-100477

antibody from Invitrogen Antibodies

Targeting: DMXL2 DFNA71, KIAA0856, RC3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-100477

Provider

Invitrogen Antibodies

Product name

DMXL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total DMXL2.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DMXL2 in HepG2 cell lysate (left lane: treated with blocking peptide). Samples were incubated with DMXL2 polyclonal antibody (Product # PA5-100477).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DMXL2 in HepG2 cell lysate (left lane: treated with blocking peptide). Samples were incubated with DMXL2 polyclonal antibody (Product # PA5-100477).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DMXL2 in mouse brain tissue lysate (left lane: treated with blocking peptide). Samples were incubated with DMXL2 polyclonal antibody (Product # PA5-100477).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of DMXL2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DMXL2 polyclonal antibody (Product # PA5-100477) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of DMXL2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DMXL2 polyclonal antibody (Product # PA5-100477) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).