Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AGT-036-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMCT1 (SLC5A8) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitution: 1 X 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human LoVo colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigmented epithelium cell line lysate (2 and 4): - 1-2. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 3-4. Anti-SMCT1 (SLC5A8) Antibody , preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain lysate (lanes 1 and 4), rat kidney membranes (lanes 2 and 5) and mouse colon membranes (3 and 6): - 1-3. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 4-6. Anti-SMCT1 (SLC5A8) Antibody , preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human LoVo colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigmented epithelium cell line lysate (2 and 4): - 1-2. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 3-4. Anti-SMCT1 (SLC5A8) Antibody , preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of SMCT1 in rat supraoptic nucleus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. SMCT1 immunoreactivity (green) was detected in neuronal profiles (arrows). B. Pre-incubation of the Antibody with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036), suppressed staining. Cell nuclei are stained with DAPI (blue).