PA5-71284

antibody from Invitrogen Antibodies

Targeting: WWC2 BOMB, FLJ22029

Provider product page for PA5-71284

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Other assay [2]

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Product number: PA5-71284 - Provider product page
Provider: Invitrogen Antibodies
Product name: WWC2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This target displays homology in the following species: Cow: 92%; Dog: 100%; Guinea Pig: 92%; Horse: 93%; Human: 100%; Mouse: 92%; Pig: 100%; Rat: 92%; Yeast: 83%
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

WWC2 protein structure - PA5-71284 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 5 Figure miR-10a targets WW and C2 domain containing 2 (WWC2) expression. A, Heat map for the top 30 differentially expressed genes in GSE22780 microarray. B, Combination of the results from TargetScan and mirDIP predictions and differentially expressed genes in GSE22780 microarray, and the intersection, WWC2, was found. C, Binding sites of miR-10a and WWC2 predicted on http://34.236.212.39/microrna/home.do . D, Target relation between miR-10a and WWC2 validated by dual luciferase reporter gene assay. E, F, mRNA expression (E) and protein level (F) of WWC2 PANC-1 CSCs following miR-10a intervention detected using RT-qPCR and Western blot analysis, respectively. G, H, mRNA expression (G) and protein levels (H) of WWC2 in PANC-1 CSCs, PAMC-1, and HPDE6-C7 detected using RT-qPCR and Western blot analysis, respectively. Measurement data were expressed as mean +- SD. Differences between every group pair were analyzed using independent samples t test, while differences among multiple groups were analyzed using one-way ANOVA and Tukey's multiple comparison test. *, compared to the control group, P < .05; repetition = 3. ANOVA, analysis of variance; miR-10a, microRNA-10a; mRNA, messenger RNA; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SD, standard derivation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 6 Figure miR-10a promotes EMT and stemness maintenance of PANC-1 CSCs by inhibiting WWC2 expression. A, Proliferation activity of PANC-1 CSCs detected using EdU assay (x200). B, Invasion and migration abilities of PANC-1 CSCs measured using Transwell assays (x200). C, Tumor formation ability of PANC-1 CSCs detected via self-renewal assay. D, Colony formation ability of PANC-1 CSCs assessed using colony formation assay in soft agar. E, Expression of miR-10a and stem cancer biomarkers (CD44, CD24, EpCAM, Nanog, OCT-4, and SOX-2) in PANC-1 CSCs evaluated using RT-qPCR. F, Protein levels of EMT-related proteins (E-cadherin, N-cadherin, and Vimentin) and stemness-related proteins (CD44, CD24, EpCAM, Nanog, OCT-4, and SOX-2) detected using Western blot analysis. Measurement data were expressed as mean +- SD. Differences between every group pair were analyzed using independent samples t test, while differences among multiple groups were analyzed using one-way ANOVA and Tukey's multiple comparison test. *, compared to the control group, P < .05; repetition = 3. ANOVA, analysis of variance; CSC, cancer stem cell; EdU, 5-ethynyl-2'-deoxyuridine; EMT, epithelial-to-mesenchymal transition; EpCAM, epithelial cellular adhesion molecule; miR-10a, microRNA-10a; OCT-4, octamer-binding transcription factor-4; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SD, standard derivation; SOX-2, SRY-related high-mobility-group (HMG)-box protein-2; WWC2, WW and C2 domain containi