PAB27927

antibody from Abnova Corporation

Targeting: MVB12A FAM125A, FLJ32495

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27927

Provider

Abnova Corporation

Product name

FAM125A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant FAM125A.

Antigen sequence

ISCTVEGAPASFGKSFAQKSGYFLCLSSLGSLENP
QENVVADIQIVVDKSPLPLGFSPVCDPMDSKASVS
KKKRMCVKLLPL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with FAM125A polyclonal antibody (Cat # PAB27927) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line HEK 293 with FAM125A polyclonal antibody (Cat # PAB27927) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and aggresome.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human bronchus with FAM125A polyclonal antibody (Cat # PAB27927) shows strong cytoplasmic and membranous positivity in respiratory epithelial cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)