PAB27916

antibody from Abnova Corporation

Targeting: MVB12A FAM125A, FLJ32495

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27916

Provider

Abnova Corporation

Product name

FAM125A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant FAM125A.

Antigen sequence

LGATDTAVFDVRLSGKTKTVPGYLRIGDMGGFAIW
CKKAKAPRPVPKPRGLSRDMQGLSLDAASQPSKGG
LLERTASRLGSRASTL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with FAM125A polyclonal antibody (Cat # PAB27916) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with FAM125A polyclonal antibody (Cat # PAB27916) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with FAM125A polyclonal antibody (Cat # PAB27916) shows moderate cytoplasmic and membranous positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)