- HPA023374 - Atlas Antibodies PCM1 antibody

Submitted references BBS mutations modify phenotypic expression of CEP290-related ciliopathies.

Zhang Y, Seo S, Bhattarai S, Bugge K, Searby CC, Zhang Q, Drack AV, Stone EM, Sheffield VC
Human molecular genetics 2014 Jan 1;23(1):40-51

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Humbert MC, Weihbrecht K, Searby CC, Li Y, Pope RM, Sheffield VC, Seo S
Proceedings of the National Academy of Sciences of the United States of America 2012 Nov 27;109(48):19691-6

Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover

Bergmann O, Zdunek S, Alkass K, Druid H, Bernard S, Frisén J
Experimental Cell Research 2011 January;317(2):188-194

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane & centrosome.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human testis and liver tissues using HPA023374 antibody. Corresponding PCM1 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human kidney, liver, ovary and testis using Anti-PCM1 antibody HPA023374 (A) shows similar protein distribution across tissues to independent antibody HPA023370 (B).
Antibody #2 product nr: HPA023370
Antibody provider: Atlas Antibodies

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in a subset of glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human ovary shows strong positivity in oocyte.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver shows weak positivity.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human ovary using Anti-PCM1 antibody HPA023374.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis using Anti-PCM1 antibody HPA023374.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver using Anti-PCM1 antibody HPA023374.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-PCM1 antibody HPA023374.
Sample type: HUMAN

HPA023374

Antibody data