HPA047921

antibody from Atlas Antibodies

Targeting: THOC2 CXorf3, dJ506G2.1, MRX12, MRX35, THO2

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA047921

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA047921, RRID:AB_10960388

Product name

Anti-THOC2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VHKWHYKLTKASVHCLETGEYTHIRNILIVLTKIL
PWYPKVLNLGQALERRVHKICQEEKEKRPDLYALA
MGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASV


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

klas2

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting THOC2 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting THOC2 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control

Sample type

U-2 OS

Primary Ab dilution

1:156

Conjugate

Horseradish Peroxidase

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:3000

Knockdown/Genetic Approaches Application

Western blot

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no

s32860

KD Reagent Provider

Thermo Fisher Scientific

KD Reagent Prod no 2

s32861

KD Reagent Page 2

KD Reagent Page 2

KD Reagent Prod no 3

s32862

KD Reagent Page 3

KD Reagent Page 3

Antibody Lot Number

R45098

Appendix

566c64a943650.pdf

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-THOC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HEL.

Sample type

HUMAN

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human appendix shows strong nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and glomeruli.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows strong nuclear positivity in squamous epithelial cells.

Sample type

HUMAN