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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB6860 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NEU-1/Sialidase-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NEU-1/Sialidase-1 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q99519- Isotype
- IgG
- Antibody clone number
- 688215
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Sialic acid attenuates puromycin aminonucleoside-induced desialylation and oxidative stress in human podocytes.
Pawluczyk IZ, Ghaderi Najafabadi M, Patel S, Desai P, Vashi D, Saleem MA, Topham PS
Experimental cell research 2014 Jan 15;320(2):258-68
Experimental cell research 2014 Jan 15;320(2):258-68
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human NEU-1/Sialidase-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NEU-1/Sialidase-1 Monoclonal Antibody (Catalog # MAB6860) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEU-1/Sialidase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
