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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- A302-709A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIF7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human KIF7 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, RKO, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-KIF7 antibody (Product # A302-709A; lot A302-709A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human KIF7 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-KIF7 antibody A302-707A used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human KIF7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-KIF7 antibody (Product # A302-709A; lot A302-709A-2) used for IP at 6 µg per reaction. KIF7 was also immunoprecipitated by a previous lot of this antibody (lot A302-709A-1) and rabbit anti-KIF7 antibody BL6860. For blotting immunoprecipitated KIF7, Product # A302-709A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
