Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-46838 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46838, RRID:AB_10009901
- Product name
- Rabbit Polyclonal KIF7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KIF7 Antibody [NBP1-46838] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibody used at 0.4 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KIF7 Antibody [NBP1-46838] - Detection of human KIF7 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, RKO, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-KIF7 antibody NBP1-46838 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: KIF7 Antibody [NBP1-46838] - Detection of human KIF7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-KIF7 antibody NBP1-46838 (lot NBP1-46838-2) used for IP at 6 ug per reaction. KIF7 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46838-1) and another rabbit anti-KIF7 antibody. For blotting immunoprecipitated KIF7, NBP1-46838 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.