PA5-118310

antibody from Invitrogen Antibodies

Targeting: TIMM29 C19orf52, TIM29

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118310

Provider

Invitrogen Antibodies

Product name

C19orf52 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using C19orf52 Polyclonal Antibody (Product # PA5-118310) at 1:500 dilution. Lane A: RT4 Whole Cell Lysate, Lane B: U-251 MG Whole Cell Lysate, Lane C: NIH/3T3 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of C19orf52 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with C19orf52 Polyclonal Antibody (Product # PA5-118310, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C19orf52 Immunoprecipitation using: Lane A: 0.5 mg RT-4 Whole Cell Lysate 4 µL with C19orf52 Polyclonal Antibody (Product # PA5-118310) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: C19orf52 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa.