Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-82612 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82612, RRID:AB_11036137
- Product name
- Rabbit Polyclonal MTHFD1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat MTHFD1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Cancer cell response to anthracyclines effects: mysteries of the hidden proteins associated with these drugs.
Tyleckova J, Hrabakova R, Mairychova K, Halada P, Radova L, Dzubak P, Hajduch M, Gadher SJ, Kovarova H
International journal of molecular sciences 2012 Nov 22;13(12):15536-64
International journal of molecular sciences 2012 Nov 22;13(12):15536-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using anti-MTHFD1 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis using Anti-MTHFD1 antibody NBP1-82612 (A) shows similar pattern to independent antibody NBP1-82611 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.