Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF6795 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Protogenin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Protegenin in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Protegenin is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q2VWP7
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Protogenin in Mouse Embryo. Protogenin was detected in immersion fixed frozen sections of mouse embryo (E9.5) using Sheep Anti-Human Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6795) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to nuclei in the neural tube. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.