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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 730050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SFMBT2 Monoclonal Antibody, Biotin
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Submitted references Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, Byram G, Collins BC, Dowdell E, Fenner M, Guo X, Hutchinson A, Kennedy JJ, Krastins B, Larsen B, Lin ZY, Lopez MF, Loppnau P, Miersch S, Nguyen T, Olsen JB, Paduch M, Ravichandran M, Seitova A, Vadali G, Vogelsang MS, Whiteaker JR, Zhong G, Zhong N, Zhao L, Aebersold R, Arrowsmith CH, Emili A, Frappier L, Gingras AC, Gstaiger M, Paulovich AG, Koide S, Kossiakoff AA, Sidhu SS, Wodak SJ, Gräslund S, Greenblatt JF, Edwards AM
Nature methods 2015 Aug;12(8):725-31
Nature methods 2015 Aug;12(8):725-31
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SFMBT2 in Hela and 293T Cells using Anti-SFMBT2 Recombinant Mouse Monoclonal Antibody (Product # 730050). Cells were fixed with 3% Formaldehyde and lysed with 1% TritonX-100. Hela cells or 293T Cells were then single labeled with Anti-SFMBT2 Recombinant Mouse Monoclonal Antibody (Product # 730050) and detected with Goat-anti-human Alexa Fluor dye F488 (green). FLAG cells were double labeled with human Fab and detected with Goat-anti-human Alexa Fluor dye F488 (green) as well as with Mouse-anti-FLAG-M2 and detected with Goat-anti-Mouse Alexa Fluor dye F647 (yellow). The DNA in the nucleus was detected with DAPI (Blue). Control means cells were labeled only with secondary antibody Alexa Fluor Dye.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SFMBT2 in Hela and 293T Cells using Anti-SFMBT2 Recombinant Mouse Monoclonal Antibody (Product # 730050). Cells were fixed with 3% Formaldehyde and lysed with 1% TritonX-100. Hela cells or 293T Cells were then single labeled with Anti-SFMBT2 Recombinant Mouse Monoclonal Antibody (Product # 730050) and detected with Goat-anti-human Alexa Fluor dye F488 (green). FLAG cells were double labeled with human Fab and detected with Goat-anti-human Alexa Fluor dye F488 (green) as well as with Mouse-anti-FLAG-M2 and detected with Goat-anti-Mouse Alexa Fluor dye F647 (yellow). The DNA in the nucleus was detected with DAPI (Blue). Control means cells were labeled only with secondary antibody Alexa Fluor Dye.
- Conjugate
- Biotin
