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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-104160 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC9A10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total SLC9A10.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references The sodium/proton exchanger SLC9C1 (sNHE) is essential for human sperm motility and fertility.
Cavarocchi E, Whitfield M, Chargui A, Stouvenel L, Lorès P, Coutton C, Arnoult C, Santulli P, Patrat C, Thierry-Mieg N, Ray PF, Dulioust E, Touré A
Clinical genetics 2021 May;99(5):684-693
Clinical genetics 2021 May;99(5):684-693
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC9A10 in HepG2 cell lysate. Samples were incubated with SLC9A10 polyclonal antibody (Product # PA5-104160).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3 FIGURE Analysis of SLC9C1 protein in sperm from the patient carrying the c.2748 + 2 T > C mutation. (A) SLC9C1 protein detection (red) by immunofluorescence assay in sperm cells from control individual (top) and patient (bottom). Two different antibodies were used: one antibody directed against the central region of SLC9C1 and a second antibody directed against the C-terminal region and including exon 22 coding region. Co-staining with alpha-tubulin antibody (green) was also performed. The protein localises at the midpiece and principal piece of sperm flagella, with a decreasing gradient towards the end of the tail. The central antibody allowed to confirm presence of SLC9C1 in patient spermatozoa, while absence of signal of the C-terminal antibody confirmed an abnormal protein lacking part of the CNBD. Scale bars represent 10 mum. (B) Immunoblotting detection of SLC9C1 protein (135 kDa) in sperm lysate from control individual (left) and patient (right). beta-tubulin (55 kDa) was used for normalization
