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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [3]
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- Product number
- PA5-68358 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP51 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with Human, Mouse, and Rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1.
Zhou F, Du C, Xu D, Lu J, Zhou L, Wu C, Wu B, Huang J
Molecular medicine reports 2020 Aug;22(2):1382-1390
Molecular medicine reports 2020 Aug;22(2):1382-1390
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP51 in COS7 whole cell lysates using a USP51 Polyclonal Antibody (Product # PA5-68358).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Expression of USP51 and ZEB1 is significantly increased in A549 or A549/DDP cells. Total RNA and protein were extracted from A549, A549/DDP and 16HBE cells. (A) After treatment with gradient concentrations of DDP (0, 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 umol/l), cell proliferation was assessed to determine drug resistance in the indicated cells (A549 and A549/DDP) to DDP. The mRNA expression of (B) USP51 and (C) ZEB1 was detected by reverse transcription-quantitative PCR. (D) Protein expression levels of USP51 and ZEB1 were detected by western blotting. **P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Knockdown or overexpression of USP51 in A549 or A549/DDP cells by lentivirus infection. A549 cells were infected with USP51/vector while A549/DDP cells were infected with shUSP51/shNC. (A and B) USP51 mRNA expression (upper) and protein levels (lower) in (A) A549 or (B) A549/DDP cells were determined by reverse transcription-quantitative PCR and western blotting, respectively. ***P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. Knockdown of USP51 decreases DDP resistance in A549/DDP cells by modulating ZEB1 ubiquitination. (A) After infection of ZEB1/vector lentivirus in A549/DDP cells, ZEB1 mRNA and protein levels were detected. (B) A549/DDP cells were infected with shUSP51 and ZEB1 lentiviruses and treated with 100 umol/l DDP, and apoptotic cells were detected. (C) ZEB1 mRNA expression in USP51-silenced A549/DDP cells was detected. (D) Interaction between USP51 and ZEB1 was determined by Co-Immunoprecipitation experiment. (E) Ubiquitin-mediated degradation of ZEB1 in USP51-silenced A549/DDP cells, as well as Zeb1 and USP51 protein, was examined by western blotting. *P
