Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00001937-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001937-M01, RRID:AB_2097148
- Product name
- Mouse Monoclonal EEF1G Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. EEF1G - eukaryotic translation elongation factor 1 gamma
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.
The eEF1γ subunit contacts RNA polymerase II and binds vimentin promoter region.
Phosphorylation of human eukaryotic elongation factor 1Bgamma is regulated by paclitaxel.
Blandin G, Marchand S, Charton K, Danièle N, Gicquel E, Boucheteil JB, Bentaib A, Barrault L, Stockholm D, Bartoli M, Richard I
Skeletal muscle 2013 Feb 15;3(1):3
Skeletal muscle 2013 Feb 15;3(1):3
The eEF1γ subunit contacts RNA polymerase II and binds vimentin promoter region.
Corbi N, Batassa EM, Pisani C, Onori A, Di Certo MG, Strimpakos G, Fanciulli M, Mattei E, Passananti C
PloS one 2010 Dec 31;5(12):e14481
PloS one 2010 Dec 31;5(12):e14481
Phosphorylation of human eukaryotic elongation factor 1Bgamma is regulated by paclitaxel.
Prado MA, Casado P, Zuazua-Villar P, del Valle E, Artime N, Cabal-Hierro L, Martínez-Campa CM, Lazo PS, Ramos S
Proteomics 2007 Sep;7(18):3299-304
Proteomics 2007 Sep;7(18):3299-304
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G over-expressed 293 cell line, cotransfected with EEF1G Validated Chimera RNAi ( Cat # H00001937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EEF1G monoclonal antibody (M01), clone 3F11-1A10 (Cat # H00001937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G expression in transfected 293T cell line by EEF1G monoclonal antibody (M01), clone 3F11-1A10.Lane 1: EEF1G transfected lysate(50 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in HepG2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10 Analysis of EEF1G expression in HL-60.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in 293.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of monoclonal antibody to EEF1G on HeLa cell. Antibody concentration 10 ug/ml.