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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP50935PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP50935PU-N, RRID:AB_11145455
- Product name
- anti C12orf29 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of Human CL029
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in mouse bladder tissue (left) and NCI-H460 cell line (right) lysates  (35ug/lane) using CL029 antibody (N-term) Cat.-No AP50935PU-N. This demonstrates the CL029 antibody detected the CL029 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis ofCL029 antibody (N-term) Cat.-No AP50935PU-N with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using CL029 antibody (N-term) Cat.-No AP50935PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CL029 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of NCI-H460 cells using CL029 antibody (N-term) Cat.-No AP50935PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
