HPA040995

antibody from Atlas Antibodies

Targeting: RMI2 BLAP18, C16orf75, MGC24665

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA040995

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA040995, RRID:AB_10795531

Product name

Anti-RMI2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

GKYVMVMGVVQACSPEPCLQAVKMTDLSDNPIHES
MWELEVEDLHRNI


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines MCF-7 and U-251MG using Anti-RMI2 antibody. Corresponding RMI2 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HepG2.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.

Sample type

HUMAN