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LearnNBP1-02456
antibody from Novus Biologicals
Targeting: PRKDC
DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-02456 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-02456, RRID:AB_1522176
- Product name
- Rabbit Polyclonal DNA-PKcs Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. aa 2604-2614 of human DNA PKcs.
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Epidermal growth factor receptor-dependent DNA repair promotes murine and human hematopoietic regeneration.
Accumulation of DNA damage-induced chromatin alterations in tissue-specific stem cells: the driving force of aging?
Beyond repair foci: DNA double-strand break repair in euchromatic and heterochromatic compartments analyzed by transmission electron microscopy.
Fang T, Zhang Y, Chang VY, Roos M, Termini CM, Signaevskaia L, Quarmyne M, Lin PK, Pang A, Kan J, Yan X, Javier A, Pohl K, Zhao L, Scott P, Himburg HA, Chute JP
Blood 2020 Jul 23;136(4):441-454
Blood 2020 Jul 23;136(4):441-454
Accumulation of DNA damage-induced chromatin alterations in tissue-specific stem cells: the driving force of aging?
Schuler N, Rübe CE
PloS one 2013;8(5):e63932
PloS one 2013;8(5):e63932
Beyond repair foci: DNA double-strand break repair in euchromatic and heterochromatic compartments analyzed by transmission electron microscopy.
Lorat Y, Schanz S, Schuler N, Wennemuth G, Rübe C, Rübe CE
PloS one 2012;7(5):e38165
PloS one 2012;7(5):e38165
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Detection of a 460 kD band corresponding to human DNA-PKcs in various preparations. Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNA-PKcs inhibitor + IR, Lane 4: MO59J ( DNA-PKcs-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNA-PKcs inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Staining of human prostate tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
