H00005591-M02
antibody from Abnova Corporation
Targeting: PRKDC
DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00005591-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005591-M02, RRID:AB_733243
- Product name
- PRKDC monoclonal antibody (M02), clone 1B9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PRKDC.
- Antigen sequence
KMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAG
ANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHN
IRAQEPESGLSEETQVKCLMDQATDPNILGRTWEG
REPWM- Isotype
- IgG
- Antibody clone number
- 1B9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Quantification of the host response proteome after herpes simplex virus type 1 infection.
Berard AR, Coombs KM, Severini A
Journal of proteome research 2015 May 1;14(5):2121-42
Journal of proteome research 2015 May 1;14(5):2121-42
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PRKDC monoclonal antibody (M02), clone 1B9 Western Blot analysis of PRKDC expression in A-431 ( Cat # L015V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PRKDC is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol