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LearnMA5-13404
antibody from Invitrogen Antibodies
Targeting: PRKDC
DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7
Western blot
Gel shiftAntibody data
- Antibody Data
- Antigen structure
- References [87]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-13404 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DNA-PK Antibody Cocktail
- Antibody type
- Other
- Antigen
- Purifed from natural sources
- Description
- MA5-13404 targets DNA-PKcs in gel shift, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and western blot applications and shows reactivity with Chicken, Hamster, Human, mouse, and Xenopus laevis samples. The MA5-13404 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.
- Reactivity
- Human, Mouse, Chicken/Avian, Hamster, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 18-2 + 25-4 + 42-psc
- Vial size
- 500 µL
- Concentration
- 0.2 mg/ml
- Storage
- 4° C
Submitted references Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer.
A new phosphorylated form of Ku70 identified in resistant leukemic cells confers fast but unfaithful DNA repair in cancer cell lines.
The combination of Hsp90 inhibitor 17AAG and heavy-ion irradiation provides effective tumor control in human lung cancer cells.
Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers.
Phosphorylated Sp1 is the regulator of DNA-PKcs and DNA ligase IV transcription of daunorubicin-resistant leukemia cell lines.
C/EBPβ (CCAAT/enhancer-binding protein β) mediates progesterone production through transcriptional regulation in co-operation with SF-1 (steroidogenic factor-1).
DNA-PKcs is required to maintain stability of Chk1 and Claspin for optimal replication stress response.
Nuclear survivin and its relationship to DNA damage repair genes in non-small cell lung cancer investigated using tissue array.
Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.
Involvement of DNA-PKcs in the IL-6 and IL-12 response to CpG-ODN is mediated by its interaction with TRAF6 in dendritic cells.
siRNA screening identifies differences in the Fanconi anemia pathway in BALB/c-Trp53+/- with susceptibility versus C57BL/6-Trp53+/- mice with resistance to mammary tumors.
Identification of DNA-dependent protein kinase catalytic subunit as a novel interaction partner of lymphocyte enhancer factor 1.
Phosphorylation of the cryptochrome 1 C-terminal tail regulates circadian period length.
DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1.
Inhibition of DNA damage repair by artificial activation of PARP with siDNA.
The RNA-binding protein fused in sarcoma (FUS) functions downstream of poly(ADP-ribose) polymerase (PARP) in response to DNA damage.
Genomic instability and telomere fusion of canine osteosarcoma cells.
p21 promotes error-free replication-coupled DNA double-strand break repair.
Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts.
Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.
NF-κB regulates DNA double-strand break repair in conjunction with BRCA1-CtIP complexes.
Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity.
Modification of the DNA damage response by therapeutic CDK4/6 inhibition.
Unmodified histone H3K4 and DNA-dependent protein kinase recruit autoimmune regulator to target genes.
Microsatellite instability in pediatric high grade glioma is associated with genomic profile and differential target gene inactivation.
Gene amplification in human cells knocked down for RAD54.
A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia.
The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival.
Ataxia telangiectasia mutated (Atm) and DNA-PKcs kinases have overlapping activities during chromosomal signal joint formation.
Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.
Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.
Regulatory roles of tankyrase 1 at telomeres and in DNA repair: suppression of T-SCE and stabilization of DNA-PKcs.
MAb 806 enhances the efficacy of ionizing radiation in glioma xenografts expressing the de2-7 epidermal growth factor receptor.
Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice.
Defective Artemis causes mild telomere dysfunction.
Transcription-dependent activation of ataxia telangiectasia mutated prevents DNA-dependent protein kinase-mediated cell death in response to topoisomerase I poison.
A novel splice variant of the DNA-PKcs gene is associated with clinical and cellular radiosensitivity in a patient with xeroderma pigmentosum.
Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin.
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
ARTEMIS nuclease facilitates apoptotic chromatin cleavage.
Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway.
Induction of ERBB2 nuclear transport after radiation in breast cancer cells.
DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression.
DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms.
Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression.
Positive regulation of DNA double strand break repair activity during differentiation of long life span cells: the example of adipogenesis.
Characterization of a cancer cell line that expresses a splicing variant form of 53BP1: separation of checkpoint and repair functions in 53BP1.
Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.
DNA-dependent protein kinase is involved in heat shock protein-mediated accumulation of hypoxia-inducible factor-1alpha in hypoxic preconditioned HepG2 cells.
Sulforaphane induces DNA double strand breaks predominantly repaired by homologous recombination pathway in human cancer cells.
Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo.
Analytical biochemistry of DNA--protein assemblies from crude cell extracts.
Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell.
Phosphorylation of RNA helicase A by DNA-dependent protein kinase is indispensable for expression of the MDR1 gene product P-glycoprotein in multidrug-resistant human leukemia cells.
Somatic mutations in the tyrosine kinase domain of epidermal growth factor receptor (EGFR) abrogate EGFR-mediated radioprotection in non-small cell lung carcinoma.
Coupling caspase cleavage and ubiquitin-proteasome-dependent degradation of SSRP1 during apoptosis.
The FATC domains of PIKK proteins are functionally equivalent and participate in the Tip60-dependent activation of DNA-PKcs and ATM.
Inhibition of homologous recombination repair in irradiated tumor cells pretreated with Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin.
DNA end joining activity is reduced in Alzheimer's disease.
Suppression of HIV-1 infection by a small molecule inhibitor of the ATM kinase.
Tyrosine kinase inhibitor AG1024 exerts antileukaemic effects on STI571-resistant Bcr-Abl expressing cells and decreases AKT phosphorylation.
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis.
Backup pathways of NHEJ are suppressed by DNA-PK.
The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination.
DNA-dependent PK inhibits adeno-associated virus DNA integration.
Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies.
Biochemical evidence for Ku-independent backup pathways of NHEJ.
Multiple molecular mechanisms contribute to radiation sensitivity in mantle cell lymphoma.
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle.
Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment.
Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy.
Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults.
Subnuclear localization of Ku protein: functional association with RNA polymerase II elongation sites.
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
Cisplatin induces the proapoptotic conformation of Bak in a deltaMEKK1-dependent manner.
Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism.
The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.
Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.
Ku is associated with the telomere in mammals.
Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.
Zhang Y, He Q, Hu Z, Feng Y, Fan L, Tang Z, Yuan J, Shan W, Li C, Hu X, Tanyi JL, Fan Y, Huang Q, Montone K, Dang CV, Zhang L
Nature structural & molecular biology 2016 Jun;23(6):522-30
Nature structural & molecular biology 2016 Jun;23(6):522-30
A new phosphorylated form of Ku70 identified in resistant leukemic cells confers fast but unfaithful DNA repair in cancer cell lines.
Bouley J, Saad L, Grall R, Schellenbauer A, Biard D, Paget V, Morel-Altmeyer S, Guipaud O, Chambon C, Salles B, Maloum K, Merle-Béral H, Chevillard S, Delic J
Oncotarget 2015 Sep 29;6(29):27980-8000
Oncotarget 2015 Sep 29;6(29):27980-8000
The combination of Hsp90 inhibitor 17AAG and heavy-ion irradiation provides effective tumor control in human lung cancer cells.
Hirakawa H, Fujisawa H, Masaoka A, Noguchi M, Hirayama R, Takahashi M, Fujimori A, Okayasu R
Cancer medicine 2015 Mar;4(3):426-36
Cancer medicine 2015 Mar;4(3):426-36
Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers.
He S, Zhao Z, Yang Y, O'Connell D, Zhang X, Oh S, Ma B, Lee JH, Zhang T, Varghese B, Yip J, Dolatshahi Pirooz S, Li M, Zhang Y, Li GM, Ellen Martin S, Machida K, Liang C
Nature communications 2015 Aug 3;6:7839
Nature communications 2015 Aug 3;6:7839
Phosphorylated Sp1 is the regulator of DNA-PKcs and DNA ligase IV transcription of daunorubicin-resistant leukemia cell lines.
Nishida Y, Mizutani N, Inoue M, Omori Y, Tamiya-Koizumi K, Takagi A, Kojima T, Suzuki M, Nozawa Y, Minami Y, Ohnishi K, Naoe T, Murate T
Biochimica et biophysica acta 2014;1839(4):265-74
Biochimica et biophysica acta 2014;1839(4):265-74
C/EBPβ (CCAAT/enhancer-binding protein β) mediates progesterone production through transcriptional regulation in co-operation with SF-1 (steroidogenic factor-1).
Mizutani T, Ju Y, Imamichi Y, Osaki T, Yazawa T, Kawabe S, Ishikane S, Matsumura T, Kanno M, Kamiki Y, Kimura K, Minamino N, Miyamoto K
The Biochemical journal 2014 Jun 15;460(3):459-71
The Biochemical journal 2014 Jun 15;460(3):459-71
DNA-PKcs is required to maintain stability of Chk1 and Claspin for optimal replication stress response.
Lin YF, Shih HY, Shang Z, Matsunaga S, Chen BP
Nucleic acids research 2014 Apr;42(7):4463-73
Nucleic acids research 2014 Apr;42(7):4463-73
Nuclear survivin and its relationship to DNA damage repair genes in non-small cell lung cancer investigated using tissue array.
Hu S, Qu Y, Xu X, Xu Q, Geng J, Xu J
PloS one 2013;8(9):e74161
PloS one 2013;8(9):e74161
Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.
Cousin W, Ho ML, Desai R, Tham A, Chen RY, Kung S, Elabd C, Conboy IM
PloS one 2013;8(5):e63528
PloS one 2013;8(5):e63528
Involvement of DNA-PKcs in the IL-6 and IL-12 response to CpG-ODN is mediated by its interaction with TRAF6 in dendritic cells.
Ma C, Muranyi M, Chu CH, Zhang J, Chu WM
PloS one 2013;8(3):e58072
PloS one 2013;8(3):e58072
siRNA screening identifies differences in the Fanconi anemia pathway in BALB/c-Trp53+/- with susceptibility versus C57BL/6-Trp53+/- mice with resistance to mammary tumors.
Böhringer M, Obermeier K, Griner N, Waldraff D, Dickinson E, Eirich K, Schindler D, Hagen M, Jerry DJ, Wiesmüller L
Oncogene 2013 Nov 28;32(48):5458-70
Oncogene 2013 Nov 28;32(48):5458-70
Identification of DNA-dependent protein kinase catalytic subunit as a novel interaction partner of lymphocyte enhancer factor 1.
Shimomura A, Takasaki A, Nomura R, Hayashi N, Senda T
Medical molecular morphology 2013 Mar;46(1):14-9
Medical molecular morphology 2013 Mar;46(1):14-9
Phosphorylation of the cryptochrome 1 C-terminal tail regulates circadian period length.
Gao P, Yoo SH, Lee KJ, Rosensweig C, Takahashi JS, Chen BP, Green CB
The Journal of biological chemistry 2013 Dec 6;288(49):35277-86
The Journal of biological chemistry 2013 Dec 6;288(49):35277-86
DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1.
Calkins AS, Iglehart JD, Lazaro JB
Nucleic acids research 2013 Aug;41(15):7378-86
Nucleic acids research 2013 Aug;41(15):7378-86
Inhibition of DNA damage repair by artificial activation of PARP with siDNA.
Croset A, Cordelières FP, Berthault N, Buhler C, Sun JS, Quanz M, Dutreix M
Nucleic acids research 2013 Aug;41(15):7344-55
Nucleic acids research 2013 Aug;41(15):7344-55
The RNA-binding protein fused in sarcoma (FUS) functions downstream of poly(ADP-ribose) polymerase (PARP) in response to DNA damage.
Mastrocola AS, Kim SH, Trinh AT, Rodenkirch LA, Tibbetts RS
The Journal of biological chemistry 2013 Aug 23;288(34):24731-41
The Journal of biological chemistry 2013 Aug 23;288(34):24731-41
Genomic instability and telomere fusion of canine osteosarcoma cells.
Maeda J, Yurkon CR, Fujisawa H, Kaneko M, Genet SC, Roybal EJ, Rota GW, Saffer ER, Rose BJ, Hanneman WH, Thamm DH, Kato TA
PloS one 2012;7(8):e43355
PloS one 2012;7(8):e43355
p21 promotes error-free replication-coupled DNA double-strand break repair.
Mauro M, Rego MA, Boisvert RA, Esashi F, Cavallo F, Jasin M, Howlett NG
Nucleic acids research 2012 Sep 1;40(17):8348-60
Nucleic acids research 2012 Sep 1;40(17):8348-60
Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts.
Nguyen CL, Possemato R, Bauerlein EL, Xie A, Scully R, Hahn WC
Molecular and cellular biology 2012 Oct;32(19):3963-77
Molecular and cellular biology 2012 Oct;32(19):3963-77
Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.
Kang KB, Zhu C, Wong YL, Gao Q, Ty A, Wong MC
International journal of radiation oncology, biology, physics 2012 May 1;83(1):e43-52
International journal of radiation oncology, biology, physics 2012 May 1;83(1):e43-52
NF-κB regulates DNA double-strand break repair in conjunction with BRCA1-CtIP complexes.
Volcic M, Karl S, Baumann B, Salles D, Daniel P, Fulda S, Wiesmüller L
Nucleic acids research 2012 Jan;40(1):181-95
Nucleic acids research 2012 Jan;40(1):181-95
Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity.
Cardinale A, Racaniello M, Saladini S, De Chiara G, Mollinari C, de Stefano MC, Pocchiari M, Garaci E, Merlo D
The Journal of biological chemistry 2012 Jan 20;287(4):2618-31
The Journal of biological chemistry 2012 Jan 20;287(4):2618-31
Modification of the DNA damage response by therapeutic CDK4/6 inhibition.
Dean JL, McClendon AK, Knudsen ES
The Journal of biological chemistry 2012 Aug 17;287(34):29075-87
The Journal of biological chemistry 2012 Aug 17;287(34):29075-87
Unmodified histone H3K4 and DNA-dependent protein kinase recruit autoimmune regulator to target genes.
Žumer K, Low AK, Jiang H, Saksela K, Peterlin BM
Molecular and cellular biology 2012 Apr;32(8):1354-62
Molecular and cellular biology 2012 Apr;32(8):1354-62
Microsatellite instability in pediatric high grade glioma is associated with genomic profile and differential target gene inactivation.
Viana-Pereira M, Lee A, Popov S, Bax DA, Al-Sarraj S, Bridges LR, Stávale JN, Hargrave D, Jones C, Reis RM
PloS one 2011;6(5):e20588
PloS one 2011;6(5):e20588
Gene amplification in human cells knocked down for RAD54.
Ruiz-Herrera A, Smirnova A, Khoriauli L, Nergadze SG, Mondello C, Giulotto E
Genome integrity 2011 Mar 18;2(1):5
Genome integrity 2011 Mar 18;2(1):5
A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia.
Bouquet F, Ousset M, Biard D, Fallone F, Dauvillier S, Frit P, Salles B, Muller C
Journal of cell science 2011 Jun 1;124(Pt 11):1943-51
Journal of cell science 2011 Jun 1;124(Pt 11):1943-51
The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival.
Yu X, Wang H, Wang P, Chen BP, Wang Y
Journal of cellular physiology 2011 Feb;226(2):369-74
Journal of cellular physiology 2011 Feb;226(2):369-74
Ataxia telangiectasia mutated (Atm) and DNA-PKcs kinases have overlapping activities during chromosomal signal joint formation.
Gapud EJ, Dorsett Y, Yin B, Callen E, Bredemeyer A, Mahowald GK, Omi KQ, Walker LM, Bednarski JJ, McKinnon PJ, Bassing CH, Nussenzweig A, Sleckman BP
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 1;108(5):2022-7
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 1;108(5):2022-7
Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.
Cheng Q, Barboule N, Frit P, Gomez D, Bombarde O, Couderc B, Ren GS, Salles B, Calsou P
Nucleic acids research 2011 Dec;39(22):9605-19
Nucleic acids research 2011 Dec;39(22):9605-19
Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.
Takai H, Xie Y, de Lange T, Pavletich NP
Genes & development 2010 Sep 15;24(18):2019-30
Genes & development 2010 Sep 15;24(18):2019-30
Regulatory roles of tankyrase 1 at telomeres and in DNA repair: suppression of T-SCE and stabilization of DNA-PKcs.
Dregalla RC, Zhou J, Idate RR, Battaglia CL, Liber HL, Bailey SM
Aging 2010 Oct;2(10):691-708
Aging 2010 Oct;2(10):691-708
MAb 806 enhances the efficacy of ionizing radiation in glioma xenografts expressing the de2-7 epidermal growth factor receptor.
Johns TG, McKay MJ, Cvrljevic AN, Gan HK, Taylor C, Xu H, Smyth FE, Scott AM
International journal of radiation oncology, biology, physics 2010 Oct 1;78(2):572-8
International journal of radiation oncology, biology, physics 2010 Oct 1;78(2):572-8
Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice.
Papeta N, Zheng Z, Schon EA, Brosel S, Altintas MM, Nasr SH, Reiser J, D'Agati VD, Gharavi AG
The Journal of clinical investigation 2010 Nov;120(11):4055-64
The Journal of clinical investigation 2010 Nov;120(11):4055-64
Defective Artemis causes mild telomere dysfunction.
Yasaei H, Slijepcevic P
Genome integrity 2010 May 26;1(1):3
Genome integrity 2010 May 26;1(1):3
Transcription-dependent activation of ataxia telangiectasia mutated prevents DNA-dependent protein kinase-mediated cell death in response to topoisomerase I poison.
Sakasai R, Teraoka H, Takagi M, Tibbetts RS
The Journal of biological chemistry 2010 May 14;285(20):15201-8
The Journal of biological chemistry 2010 May 14;285(20):15201-8
A novel splice variant of the DNA-PKcs gene is associated with clinical and cellular radiosensitivity in a patient with xeroderma pigmentosum.
Abbaszadeh F, Clingen PH, Arlett CF, Plowman PN, Bourton EC, Themis M, Makarov EM, Newbold RF, Green MH, Parris CN
Journal of medical genetics 2010 Mar;47(3):176-81
Journal of medical genetics 2010 Mar;47(3):176-81
Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin.
Sakasai R, Teraoka H, Tibbetts RS
DNA repair 2010 Jan 2;9(1):76-82
DNA repair 2010 Jan 2;9(1):76-82
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
Choi YK, Nash K, Byrne BJ, Muzyczka N, Song S
PloS one 2010 Dec 20;5(12):e15073
PloS one 2010 Dec 20;5(12):e15073
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
Velkova A, Carvalho MA, Johnson JO, Tavtigian SV, Monteiro AN
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.
Velkova A, Carvalho MA, Johnson JO, Tavtigian SV, Monteiro AN
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
Cell cycle (Georgetown, Tex.) 2010 Apr 1;9(7):1421-33
ARTEMIS nuclease facilitates apoptotic chromatin cleavage.
Britton S, Frit P, Biard D, Salles B, Calsou P
Cancer research 2009 Oct 15;69(20):8120-6
Cancer research 2009 Oct 15;69(20):8120-6
Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway.
Daya S, Cortez N, Berns KI
Journal of virology 2009 Nov;83(22):11655-64
Journal of virology 2009 Nov;83(22):11655-64
Induction of ERBB2 nuclear transport after radiation in breast cancer cells.
Luo B, Yu S, Zhuang L, Xia S, Zhao Z, Rong L
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2009 Jun;29(3):350-3
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2009 Jun;29(3):350-3
DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression.
Carlessi L, De Filippis L, Lecis D, Vescovi A, Delia D
Cell death and differentiation 2009 Jun;16(6):795-806
Cell death and differentiation 2009 Jun;16(6):795-806
DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms.
Choul-li S, Drobecq H, Aumercier M
Biochemical and biophysical research communications 2009 Dec 18;390(3):839-44
Biochemical and biophysical research communications 2009 Dec 18;390(3):839-44
Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression.
Ohno M, Kunimoto M, Nishizuka M, Osada S, Imagawa M
Biochemical and biophysical research communications 2009 Dec 18;390(3):738-42
Biochemical and biophysical research communications 2009 Dec 18;390(3):738-42
Positive regulation of DNA double strand break repair activity during differentiation of long life span cells: the example of adipogenesis.
Meulle A, Salles B, Daviaud D, Valet P, Muller C
PloS one 2008;3(10):e3345
PloS one 2008;3(10):e3345
Characterization of a cancer cell line that expresses a splicing variant form of 53BP1: separation of checkpoint and repair functions in 53BP1.
Iwabuchi K, Matsui T, Hashimoto M, Matsumoto Y, Kurihara T, Date T
Biochemical and biophysical research communications 2008 Nov 21;376(3):509-13
Biochemical and biophysical research communications 2008 Nov 21;376(3):509-13
Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.
Schild-Poulter C, Su A, Shih A, Kelly OP, Fritzler MJ, Goldstein R, Haché RJ
Rheumatology (Oxford, England) 2008 Feb;47(2):165-71
Rheumatology (Oxford, England) 2008 Feb;47(2):165-71
DNA-dependent protein kinase is involved in heat shock protein-mediated accumulation of hypoxia-inducible factor-1alpha in hypoxic preconditioned HepG2 cells.
Kang MJ, Jung SM, Kim MJ, Bae JH, Kim HB, Kim JY, Park SJ, Song HS, Kim DW, Kang CD, Kim SH
The FEBS journal 2008 Dec;275(23):5969-81
The FEBS journal 2008 Dec;275(23):5969-81
Sulforaphane induces DNA double strand breaks predominantly repaired by homologous recombination pathway in human cancer cells.
Sekine-Suzuki E, Yu D, Kubota N, Okayasu R, Anzai K
Biochemical and biophysical research communications 2008 Dec 12;377(2):341-345
Biochemical and biophysical research communications 2008 Dec 12;377(2):341-345
Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo.
Yu D, Sekine E, Fujimori A, Ochiya T, Okayasu R
Cancer science 2008 Apr;99(4):810-5
Cancer science 2008 Apr;99(4):810-5
Analytical biochemistry of DNA--protein assemblies from crude cell extracts.
Hégarat N, Cardoso GM, Rusconi F, Francois JC, Praseuth D
Nucleic acids research 2007;35(13):e92
Nucleic acids research 2007;35(13):e92
Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell.
Wu PY, Frit P, Malivert L, Revy P, Biard D, Salles B, Calsou P
The Journal of biological chemistry 2007 Nov 2;282(44):31937-43
The Journal of biological chemistry 2007 Nov 2;282(44):31937-43
Phosphorylation of RNA helicase A by DNA-dependent protein kinase is indispensable for expression of the MDR1 gene product P-glycoprotein in multidrug-resistant human leukemia cells.
Zhong X, Safa AR
Biochemistry 2007 May 15;46(19):5766-75
Biochemistry 2007 May 15;46(19):5766-75
Somatic mutations in the tyrosine kinase domain of epidermal growth factor receptor (EGFR) abrogate EGFR-mediated radioprotection in non-small cell lung carcinoma.
Das AK, Chen BP, Story MD, Sato M, Minna JD, Chen DJ, Nirodi CS
Cancer research 2007 Jun 1;67(11):5267-74
Cancer research 2007 Jun 1;67(11):5267-74
Coupling caspase cleavage and ubiquitin-proteasome-dependent degradation of SSRP1 during apoptosis.
Landais I, Lee H, Lu H
Cell death and differentiation 2006 Nov;13(11):1866-78
Cell death and differentiation 2006 Nov;13(11):1866-78
The FATC domains of PIKK proteins are functionally equivalent and participate in the Tip60-dependent activation of DNA-PKcs and ATM.
Jiang X, Sun Y, Chen S, Roy K, Price BD
The Journal of biological chemistry 2006 Jun 9;281(23):15741-6
The Journal of biological chemistry 2006 Jun 9;281(23):15741-6
Inhibition of homologous recombination repair in irradiated tumor cells pretreated with Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin.
Noguchi M, Yu D, Hirayama R, Ninomiya Y, Sekine E, Kubota N, Ando K, Okayasu R
Biochemical and biophysical research communications 2006 Dec 22;351(3):658-63
Biochemical and biophysical research communications 2006 Dec 22;351(3):658-63
DNA end joining activity is reduced in Alzheimer's disease.
Shackelford DA
Neurobiology of aging 2006 Apr;27(4):596-605
Neurobiology of aging 2006 Apr;27(4):596-605
Suppression of HIV-1 infection by a small molecule inhibitor of the ATM kinase.
Lau A, Swinbank KM, Ahmed PS, Taylor DL, Jackson SP, Smith GC, O'Connor MJ
Nature cell biology 2005 May;7(5):493-500
Nature cell biology 2005 May;7(5):493-500
Tyrosine kinase inhibitor AG1024 exerts antileukaemic effects on STI571-resistant Bcr-Abl expressing cells and decreases AKT phosphorylation.
Deutsch E, Maggiorella L, Wen B, Bonnet ML, Khanfir K, Frascogna V, Turhan AG, Bourhis J
British journal of cancer 2004 Nov 1;91(9):1735-41
British journal of cancer 2004 Nov 1;91(9):1735-41
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
Sawchuk DJ, Mansilla-Soto J, Alarcon C, Singha NC, Langen H, Bianchi ME, Lees-Miller SP, Nussenzweig MC, Cortes P
The Journal of biological chemistry 2004 Jul 9;279(28):29821-31
The Journal of biological chemistry 2004 Jul 9;279(28):29821-31
Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis.
Loong SL, Korzh S, Price A
Oncogene 2004 Jul 15;23(32):5562-6
Oncogene 2004 Jul 15;23(32):5562-6
Backup pathways of NHEJ are suppressed by DNA-PK.
Perrault R, Wang H, Wang M, Rosidi B, Iliakis G
Journal of cellular biochemistry 2004 Jul 1;92(4):781-94
Journal of cellular biochemistry 2004 Jul 1;92(4):781-94
The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination.
Poinsignon C, Moshous D, Callebaut I, de Chasseval R, Villey I, de Villartay JP
The Journal of experimental medicine 2004 Feb 2;199(3):315-21
The Journal of experimental medicine 2004 Feb 2;199(3):315-21
DNA-dependent PK inhibits adeno-associated virus DNA integration.
Song S, Lu Y, Choi YK, Han Y, Tang Q, Zhao G, Berns KI, Flotte TR
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2112-6
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2112-6
Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies.
Holgersson A, Erdal H, Nilsson A, Lewensohn R, Kanter L
Experimental and molecular pathology 2004 Aug;77(1):1-6
Experimental and molecular pathology 2004 Aug;77(1):1-6
Biochemical evidence for Ku-independent backup pathways of NHEJ.
Wang H, Perrault AR, Takeda Y, Qin W, Wang H, Iliakis G
Nucleic acids research 2003 Sep 15;31(18):5377-88
Nucleic acids research 2003 Sep 15;31(18):5377-88
Multiple molecular mechanisms contribute to radiation sensitivity in mantle cell lymphoma.
M'kacher R, Bennaceur A, Farace F, Laugé A, Plassa LF, Wittmer E, Dossou J, Violot D, Deutsch E, Bourhis J, Stoppa-Lyonnet D, Ribrag V, Carde P, Parmentier C, Bernheim A, Turhan AG
Oncogene 2003 Sep 11;22(39):7905-12
Oncogene 2003 Sep 11;22(39):7905-12
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
Udayakumar D, Bladen CL, Hudson FZ, Dynan WS
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Onclercq-Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor-Guéret M
Nucleic acids research 2003 Nov 1;31(21):6272-82
Nucleic acids research 2003 Nov 1;31(21):6272-82
Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle.
Lebrasseur NK, Coté GM, Miller TA, Fielding RA, Sawyer DB
American journal of physiology. Cell physiology 2003 May;284(5):C1149-55
American journal of physiology. Cell physiology 2003 May;284(5):C1149-55
Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment.
Calsou P, Delteil C, Frit P, Drouet J, Salles B
Journal of molecular biology 2003 Feb 7;326(1):93-103
Journal of molecular biology 2003 Feb 7;326(1):93-103
Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy.
Friesland S, Kanter-Lewensohn L, Tell R, Munck-Wikland E, Lewensohn R, Nilsson A
Head & neck 2003 Apr;25(4):313-21
Head & neck 2003 Apr;25(4):313-21
Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults.
Cho NH, Cordon-Cardo C, Li GC, Kim SH
The Journal of pathology 2002 Sep;198(1):21-9
The Journal of pathology 2002 Sep;198(1):21-9
Subnuclear localization of Ku protein: functional association with RNA polymerase II elongation sites.
Mo X, Dynan WS
Molecular and cellular biology 2002 Nov;22(22):8088-99
Molecular and cellular biology 2002 Nov;22(22):8088-99
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
Ma Y, Pannicke U, Schwarz K, Lieber MR
Cell 2002 Mar 22;108(6):781-94
Cell 2002 Mar 22;108(6):781-94
Cisplatin induces the proapoptotic conformation of Bak in a deltaMEKK1-dependent manner.
Mandic A, Viktorsson K, Molin M, Akusjärvi G, Eguchi H, Hayashi SI, Toi M, Hansson J, Linder S, Shoshan MC
Molecular and cellular biology 2001 Jun;21(11):3684-91
Molecular and cellular biology 2001 Jun;21(11):3684-91
Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism.
Brown KD, Lataxes TA, Shangary S, Mannino JL, Giardina JF, Chen J, Baskaran R
The Journal of biological chemistry 2000 Mar 3;275(9):6651-6
The Journal of biological chemistry 2000 Mar 3;275(9):6651-6
The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.
Jhappan C, Yusufzai TM, Anderson S, Anver MR, Merlino G
Molecular and cellular biology 2000 Jun;20(11):4075-83
Molecular and cellular biology 2000 Jun;20(11):4075-83
Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.
Chen L, Trujillo K, Sung P, Tomkinson AE
The Journal of biological chemistry 2000 Aug 25;275(34):26196-205
The Journal of biological chemistry 2000 Aug 25;275(34):26196-205
Ku is associated with the telomere in mammals.
Hsu HL, Gilley D, Blackburn EH, Chen DJ
Proceedings of the National Academy of Sciences of the United States of America 1999 Oct 26;96(22):12454-8
Proceedings of the National Academy of Sciences of the United States of America 1999 Oct 26;96(22):12454-8
Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.
Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y
The EMBO journal 1999 Mar 1;18(5):1397-406
The EMBO journal 1999 Mar 1;18(5):1397-406
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
Giffin W, Gong W, Schild-Poulter C, Haché RJ
Molecular and cellular biology 1999 Jun;19(6):4065-78
Molecular and cellular biology 1999 Jun;19(6):4065-78
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.
Gao Y, Chaudhuri J, Zhu C, Davidson L, Weaver DT, Alt FW
Immunity 1998 Sep;9(3):367-76
Immunity 1998 Sep;9(3):367-76
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of DNA-PKcs using DNA-PKcs Monoclonal Antibody (Product # MA5-13404) on LS174T Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNA-PKcs was performed by loading 30 µg of MiaPaCa-2 lysate (lane 1), 30 µg of OVCAR-8 lysate (lane 2) and 30 µg of MDA-MB-231 lysate (lane 3) in reducing sample buffer and Page Rµler Protein Ladder (Product # 26616) onto a 6% polyacrylamide gel. Proteins were transferred to PVDF membrane using the wet transfer method. Membrane was blocked in Super Block Blocking Buffer (Product # 37515). DNA-PKcs was detected at approximately 469 kDa using a DNA-PKcs monoclonal antibody (Product # MA5-13404) at a dilution of 1:1000 in 5% BSA, followed by a goat anti-mouse IgG DyLight 800 conjugated secondary antibody (Product # SA5-35521) at a dilution of 1:7500. Data courtesy of Antibody Data Exchange Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNA-PKcs was performed by loading ~300,000 cell equivalents per lane in 1X LDS reducing sample buffer (Product # NP0007) and molecular weight ladder onto a 3-8% Tris-Acetate protein gel (Product # EA0375BOX). Proteins were transferred to a PVDF membrane at 100 mA overnight. Membrane was blocked in 5% milk/TBST for 1 hour. DNA-PKcs was detected using a monoclonal antibody (Product # MA5-13404) diluted 1:1000 at 4°C, overnight. Membrane was then probed with anti-mouse secondary antibody at a dilution of 1:5000. Chemiluminescent detection was performed using Pierce ECL western blot substrate (Product # 32106). Data courtesy of the Antibody Data Exchange Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DNA-PKcs was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with DNA-PKcs (18-2 + 25-4 + 42-psc) Mouse Monoclonal Antibody (Product # MA5-13404) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175)at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of DNA-PKcs using DNA-PKcs Monoclonal Antibody (MA5-13404) on denatured Human LS174T Cells.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunoprecipitation of DNA-PKcs using DNA-PKcs Monoclonal Antibody (MA5-13404) on Native Human HUVEC Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Formalin-fixed, paraffin-embedded human testis stained with DNAKcs antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of germ cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of DNA-PKcs was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with DNA-PKcs Mouse Monoclonal Antibody (Product # MA5-13404, red histogram) or with mouse isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..
