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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 25359-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SBNO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MVEPGQDLL LAALSESGIS PNDLFDIDGG DAGLATPMPT PSVQQSVPLS ALELGLETEA AVPVKQEPET VPTPALLNVR QQPPSTTTFV LNQINHLPPL GSTIVMTKTP PVTTNRQTIT LTKFIQTTAS TRPSVSAPTV RNAMTSAPSK DQVQLKDLLK NNSLNELMKL KPPANIAQPV ATAATDVSNG TVKKESSNKE GARMWINDMK MRSFSPTMKV PVVKEDDEPE EEDEEEMGHA ETYAEYMPIK LKIGLRHPDA VVETSSLSSV TPPDVWYKTS ISEETIDNGW LSALQLEAIT Y (1-300 aa encoded by BC133704)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 25359-1-AP ( SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 25359-1-AP (SBNO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 25359-1-AP (SBNO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SBNO1 (IP:25359-1-AP, 3ug; Detection:25359-1-AP 1:300) with HeLa cells lysate 1800ug.
