PAB22965

antibody from Abnova Corporation

Targeting: ANKRD23 DARP, FLJ32449, MARP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22965

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22965, RRID:AB_11122032

Product name

ANKRD23 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ANKRD23.

Antigen sequence

KRLRHRVPPRKPEPLVKPQSQAQVEPVGLEMFLKA
AAENQEYLIDKYLTDGGDPNAHDKLHRTALHWAC


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ANKRD23 polyclonal antibody (Cat # PAB22965) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with ANKRD23 polyclonal antibody (Cat # PAB22965) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human smooth muscle with ANKRD23 polyclonal antibody (Cat # PAB22965) shows strong cytoplasmic positivity in smooth muscle cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)