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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF1067 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Growth Hormone Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Growth Hormone in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
CAA23779- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references PDK1-FoxO1 pathway in AgRP neurons of arcuate nucleus promotes bone formation via GHRH-GH-IGF1 axis.
Growth hormone is permissive for neoplastic colon growth.
STAT3 upregulation in pituitary somatotroph adenomas induces growth hormone hypersecretion.
Troubleshooting PEG-hGH detection supporting pharmacokinetic evaluation in growth hormone deficient patients.
Growth hormone is present in the human retina and vitreous fluid.
Shift of monocyte function toward cellular immunity during sleep.
Sasanuma H, Nakata M, Parmila K, Nakae J, Yada T
Molecular metabolism 2017 May;6(5):428-439
Molecular metabolism 2017 May;6(5):428-439
Growth hormone is permissive for neoplastic colon growth.
Chesnokova V, Zonis S, Zhou C, Recouvreux MV, Ben-Shlomo A, Araki T, Barrett R, Workman M, Wawrowsky K, Ljubimov VA, Uhart M, Melmed S
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 7;113(23):E3250-9
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 7;113(23):E3250-9
STAT3 upregulation in pituitary somatotroph adenomas induces growth hormone hypersecretion.
Zhou C, Jiao Y, Wang R, Ren SG, Wawrowsky K, Melmed S
The Journal of clinical investigation 2015 Apr;125(4):1692-702
The Journal of clinical investigation 2015 Apr;125(4):1692-702
Troubleshooting PEG-hGH detection supporting pharmacokinetic evaluation in growth hormone deficient patients.
Myler HA, McVay S, Kratzsch J
Journal of pharmacological and toxicological methods 2010 Mar-Apr;61(2):92-7
Journal of pharmacological and toxicological methods 2010 Mar-Apr;61(2):92-7
Growth hormone is present in the human retina and vitreous fluid.
Harvey S, Parker E, Macdonald I, Sanders EJ
Neuroscience letters 2009 May 22;455(3):199-202
Neuroscience letters 2009 May 22;455(3):199-202
Shift of monocyte function toward cellular immunity during sleep.
Lange T, Dimitrov S, Fehm HL, Westermann J, Born J
Archives of internal medicine 2006 Sep 18;166(16):1695-700
Archives of internal medicine 2006 Sep 18;166(16):1695-700
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Growth Hormone by Western Blot. Western blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Growth Hormone at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human Growth Hormone ELISA Standard Curve. Recombinant Human Growth Hormone protein was serially diluted 2-fold and captured by Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # MAB10671) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Growth Hormone in Human Pituitary. Growth Hormone was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Growth Hormone and Neutralization by Human Growth Hormone Antibody. Recombinant Human Growth Hormone (Catalog # 1067-GH) stimulates proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067). The ND50 is typically 1.5-7.5 ng/mL.
