A305-851A-T

antibody from Invitrogen Antibodies

Targeting: LSM14B bA11M20.3, C20orf40, FAM61B, FLJ25473, FT005, LSM13, RAP55B

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-851A-T

Provider

Invitrogen Antibodies

Product name

LSM14B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LSM14B by western blot. Samples: Whole cell lysate (25 µg) from Jurkat, MCF-7, U2OS, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LSM14B antibody (A305-851A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LSM14B by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, U2OS, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LSM14B antibody A305-851A-M (lot A305-851A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LSM14B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LSM14B antibody (Product # A305-851A-T; lot 2) used for IP at 6 µg per reaction. LSM14B was also immunoprecipitated by a previous lot of this antibody (Product # A305-851A-T; lot 1) and a second antibody against a different epitope of LSM14B (BL23715). For blotting immunoprecipitated LSM14B, Product # A305-851A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LSM14B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from U2OS cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LSM14B antibody (Product # A305-851A-T lot 2) used for IP at 6 µg per reaction. LSM14B was also immunoprecipitated by a previous lot of this antibody (A305-851A-T lot 1) and a second antibody against a different epitope of LSM14B (BL23715). For blotting immunoprecipitated LSM14B (Product # A305-851A-T was used at 1:1000 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.