Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00056339-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00056339-B01P, RRID:AB_2687437
- Product name
- METTL3 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human METTL3 protein.
- Antigen sequence
MSDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDL
RNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVP
ELATDPELEKKLLHHLSDLALTLPTDAVSICLAIS
TPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDA
HPTLVTYADHSKLSAMMGAVAEKKGPGEVAGTVTG
QKRRAEQDSTTVAAFASSLVSGLNSSASEPAKEPA
KKSRKHAASDVDLEIESLLNQQSTKEQQSKKVSQE
ILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGT
KEECMKASDADRPCRKLHFRRIINKHTDESLGDCS
FLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPS
QELALTQSVGGDSSADRLFPPQWICCDIRYLDVSI
LGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIP
VLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEI
IWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGN
PQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLS
PGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPD
VVARFKQRYPDGIISKPKNL- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Dynamic m(6)A mRNA methylation directs translational control of heat shock response.
Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase.
Zhou J, Wan J, Gao X, Zhang X, Jaffrey SR, Qian SB
Nature 2015 Oct 22;526(7574):591-4
Nature 2015 Oct 22;526(7574):591-4
Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase.
Ping XL, Sun BF, Wang L, Xiao W, Yang X, Wang WJ, Adhikari S, Shi Y, Lv Y, Chen YS, Zhao X, Li A, Yang Y, Dahal U, Lou XM, Liu X, Huang J, Yuan WP, Zhu XF, Cheng T, Zhao YL, Wang X, Rendtlew Danielsen JM, Liu F, Yang YG
Cell research 2014 Feb;24(2):177-89
Cell research 2014 Feb;24(2):177-89
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- METTL3 MaxPab polyclonal antibody. Western Blot analysis of METTL3 expression in human colon.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of METTL3 expression in transfected 293T cell line (H00056339-T01) by METTL3 MaxPab polyclonal antibody.Lane 1: METTL3 transfected lysate(63.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to METTL3 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol