15073-1-AP

antibody from Invitrogen Antibodies

Targeting: METTL3 M6A, MT-A70, Spo8

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15073-1-AP

Provider

Invitrogen Antibodies

Product name

METTL3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LNQQSTKEQ QSKKVSQEIL ELLNTTTAKE QSIVEKFRSR GRAQVQEFCD YGTKEECMKA SDADRPCRKL HFRRIINKHT DESLGDCSFL NTCFHMDTCK YVHYEIDACM DSEAPGSKDH TPSQELALTQ SVGGDSSADR LFPPQWICCD IRYLDVSILG KFAVVMADPP WDIHMELPYG TLTDDEMRRL NIPVLQDDGF LFLWVTGRAM ELGRECLNLW GYERVDEIIW VKTNQLQRII RTGRTGHWLN HGKEHCLVGV KGNPQGFNQG LDCDVIVAEV RSTSHKPDEI YGMIERLSPG TRKIELFGRP HNVQPNWITL GNQLDGIHLL DPDVVARFKQ RYPDGIISKP KNL (229-580 aa encoded by BC001650)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 15073-1-AP (METTL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 15073-1-AP (METTL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15073-1-AP (METTL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of METTL3 antibody (15073-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected HeLa cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 15073-1-AP (METTL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15073-1-AP (METTL3 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15073-1-AP ( METTL3 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-METTL3 (IP:15073-1-AP, 4ug; Detection:15073-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-METTL3 (IP:15073-1-AP, 4 ug; Detection:15073-1-AP 1:300) with HEK-293 cells lysate 3000 ug.