Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-77311 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77311, RRID:AB_1085116
- Product name
- Rabbit Polyclonal Methyltransferase like 3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Methyltransferase like 3 Antibody [NB100-77311] - Detection of Human METTL3 by Western Blot of immunoprecipitates. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-METTL3/MT-A70 antibody (NB100-77311) used for IP at 3 mcg/mg lysate. METTL3/MT-A70 was also immunoprecipitated by rabbit anti-METTL3/MT-A70 antibody (NB100-77310), which recognizes an upstream epitope. For blotting immunoprecipitated METTL3/MT-A70, NB100-77310 was used at 0.1 mcg/ml, and the ReliaBLOT(R) Reagents and Procedures (Bethyl Cat. No. WB120) were incorporated. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).anti-METTL3/MT-A70 antibody NB100-77310, which recognizes an upstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody [NB100-77311] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- METTL3/MT-A70 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB