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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP1-52629 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-52629, RRID:AB_11005689
- Product name
- Rabbit Polyclonal CASKIN2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CASKIN2 Antibody [NBP1-52629] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Caskin 2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Caskin 2 was also immunoprecipitated by rabbit anti-Caskin 2 antibody NBP1-52628, which recognizes an upstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CASKIN2 Antibody [NBP1-52629] - Detection of human Caskin 2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caskin 2 antibody NBP1-52629 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CASKIN2 Antibody [NBP1-52629] - Detection of human Caskin 2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Caskin 2 antibodyNBP1-52629 used for IP at 3 ug per reaction. Caskin 2 was also immunoprecipitated by rabbit anti-Caskin 2 antibody NBP1-52628. For blotting immunoprecipitated Caskin 2, NBP1-52629 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
