ABIN2616369

antibody from antibodies-online

Targeting: ANKRD24 KIAA1981

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2616369

Provider

antibodies-online

Product name

anti-Ankyrin Repeat Domain 24 (ANKRD24) (AA 296-328) antibody

Antibody type

Polyclonal

Description

Protein A purified

Reactivity

Human

Host

Rabbit

Epitope

AA 296-328

Vial size

400 μL

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 monomethyl Lys27 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys27 antibody for monomethyl Lys27 histone H3. Methylated peptides corresponding to the immunogen and related sequences derived from histone H3 were spotted onto PVDF and probed with Histone H3 monomethyl Lys27 antibody at 1 μg/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Top panel: Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9, 14, 18. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: dimethyl Lys14. Lane 10: monomethyl Lys18. Lane 11: dimethyl Lys18. Lane 12: trimethyl Lys18. Bottom panel: Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 monomethyl Lys27 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 μg) probed with Histone H3 monomethyl Lys27 antibody at a dilution of 1 μg/ml.