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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- UM500016 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500016, RRID:AB_2629031
- Product name
- SERPINB4 mouse monoclonal antibody, clone UMAB16
- Antibody type
- Monoclonal
- Description
- SERPINB4 mouse monoclonal antibody, clone UMAB16
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SERPINB4
- Isotype
- IgG
- Antibody clone number
- UMAB16
- Vial size
- 100 µl
- Concentration
- 0.50 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINB4 monoclonal antibody (Clone UMAB16) at 1:500.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:1000.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using SERPINB4 mouse monoclonal antibody (UM500016, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SERPINB4mouse monoclonal antibody. (Clone UMAB16, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-SERPINB4mouse monoclonal antibody. (Clone UMAB16, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lung tissue using anti-SERPINB4mouse monoclonal antibody. (Clone UMAB16, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-SERPINB4 clone UMAB16 mouse monoclonal antibody (UM500016) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membrane staining is seen in tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-SERPINB4 clone UMAB16 mouse monoclonal antibody (UM500016) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membrane staining is seen in tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human gastric carcinoma using anti-SERPINB4 clone UMAB16 mouse monoclonal antibody (UM500016) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membrane staining is seen in tumor cells.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SERPINB4 mouse monoclonal antibody (Clone UMAB16). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-SERPINB4 (Clone UMAB16) very specifically recognizes SERPINB4 antigen on OriGene protein microarray chip.
- Validation comment
- 10K-CHIP
