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LearnUM500016CF
antibody from Invitrogen Antibodies
Targeting: SERPINB4
LEUPIN, PI11, SCCA-2, SCCA1, SCCA2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- UM500016CF - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SERPINB4 Monoclonal Antibody (UMAB16), UltraMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB16
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Dilution: 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-SERPINB4 monoclonal antibody (Clone UMAB16) at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using SERPINB4 mouse monoclonal antibody (UM500016, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-SERPINB4mouse monoclonal antibody. (Clone UMAB16, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-SERPINB4 clone UMAB16 mouse monoclonal antibody (UM500016) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110°C. Cytoplasmic and membrane staining is seen in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-SERPINB4 clone UMAB16 mouse monoclonal antibody (UM500016) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110°C. Cytoplasmic and membrane staining is seen in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human gastric carcinoma using anti-SERPINB4 clone UMAB16 mouse monoclonal antibody (UM500016) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110°C. Cytoplasmic and membrane staining is seen in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SERPINB4mouse monoclonal antibody. (Clone UMAB16, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SERPINB4 mouse monoclonal antibody (Clone UMAB16). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-SERPINB4 (Clone UMAB16) very specifically recognizes SERPINB4 antigen on OriGene protein microarray chip.
