PAB29427

antibody from Abnova Corporation

Targeting: RCN3 RLP49

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29427

Provider

Abnova Corporation

Product name

RCN3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human RCN3.

Antigen sequence

EFPHMRDIVIAETLEDLDRNKDGYVQVEEYIADLY
SAEPGEEEPAWVQTERQQFRDFRDL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG with RCN3 polyclonal antibody (Cat # PAB29427) at 1:500-1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with RCN3 polyclonal antibody (Cat # PAB29427) at 1-4 ug/mL concentration shows positivity in endoplasmic reticulum and vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with RCN3 polyclonal antibody (Cat # PAB29427) shows strong cytoplasmic positivity in neuronal cells at 1:2500-1:5000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)