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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA5-27720 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP7A Monoclonal Antibody (S60-4)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S60-4
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon.
Rivera-Millot A, Slupek S, Chatagnon J, Roy G, Saliou JM, Billon G, Alaimo V, Hot D, Salomé-Desnoulez S, Locht C, Antoine R, Jacob-Dubuisson F
Communications biology 2021 Jan 8;4(1):46
Communications biology 2021 Jan 8;4(1):46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP7A in rat brain membrane with 20 µg of sample. The sample was blocked with 2% BSA and 2% skim milk in TBST, incubated with ATP7A monoclonal antibody (Product # MA5-27720) using a dilution of 1:1000 (16 hours at 4°C), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:100 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder (MW), Lane 2: Rat Brain membrane cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATP7A in mouse NIH 3T3 (mouse fibroblast cell line). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ATP7A monoclonal antibody (Product # MA5-27720) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Copper Transporting ATPase 1 Antibody, D) Merged image. Magnification: 60x.
