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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX115955 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115955, RRID:AB_10622324
- Product name
- Histone H2B antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Requirement for human Mps1/TTK in oxidative DNA damage repair and cell survival through MDM2 phosphorylation.
Yu ZC, Huang YF, Shieh SY
Nucleic acids research 2016 Feb 18;44(3):1133-50
Nucleic acids research 2016 Feb 18;44(3):1133-50
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE GTX115955 diluted at 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX115955) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX115955) diluted at 1:5000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Histone H2B antibody detects Histone H2B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.Green: Histone H2B protein stained by Histone H2B antibody (GTX115955) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Histone H2B(GTX115955) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Histone H2B antibody detects Histone H2B protein at nucleus in mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. Histone H2B antibody (GTX115955) diluted at 1:500.
