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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB7267A - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal TIGIT Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse TIGIT in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human TIGIT is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Binding of the Fap2 protein of Fusobacterium nucleatum to human inhibitory receptor TIGIT protects tumors from immune cell attack.
Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.
Gur C, Ibrahim Y, Isaacson B, Yamin R, Abed J, Gamliel M, Enk J, Bar-On Y, Stanietsky-Kaynan N, Coppenhagen-Glazer S, Shussman N, Almogy G, Cuapio A, Hofer E, Mevorach D, Tabib A, Ortenberg R, Markel G, Miklić K, Jonjic S, Brennan CA, Garrett WS, Bachrach G, Mandelboim O
Immunity 2015 Feb 17;42(2):344-355
Immunity 2015 Feb 17;42(2):344-355
Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.
Stanietsky N, Rovis TL, Glasner A, Seidel E, Tsukerman P, Yamin R, Enk J, Jonjic S, Mandelboim O
European journal of immunology 2013 Aug;43(8):2138-50
European journal of immunology 2013 Aug;43(8):2138-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of TIGIT in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Sheep Anti-Mouse TIGIT APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7267A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.
