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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
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- Product number
- PA5-120402 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- METT10D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: HeLa, A-549, NIH/3T3, C6, LNCaP, Rat testis. Immunogen sequence: RIQGVPKVTY TEFCQGRTMR WALAWSFYDD VTVPSPPSKR RKLEKPRKPI TFVVLASVMK ELSLKASPLR SETAEGIVVV TTWIEKILTD LKVQHKRVPC GKEEVSLFLT AIENSWIHLR RKKRERVRQL REVPRAPEDV IQALEEKKPT PKESGNSQEL ARGPQERTPC GPALREGEAA AVEGPCPSQE SLSQEENPEP TEDERSEEKG GVEVLESCQG SSNGAQDQEA SEQFGSPVAE RGKRLPGVAG QYLFKCLINV KKEVDDALVE MHWVEGQNRD LMNQLCTYIR NQIFRLVAVN
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.12 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of METT10D in LNCaP cells using a METT10D Polyclonal antibody (Product # PA5-120402) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of METT10D in various cell lines using a METT10D Polyclonal antibody (Product # PA5-120402) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of METT10D in C6 cells using a METT10D Polyclonal antibody (Product # PA5-120402). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of METT10D in C6 cells using a METT10D Polyclonal antibody (Product # PA5-120402). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of METT10D in C6 cells using a METT10D Polyclonal antibody (Product # PA5-120402). Blue: DAPI for nuclear staining.
