600-401-M75

antibody from Invitrogen Antibodies

Targeting: H3C13 HIST2H3D

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-M75

Provider

Invitrogen Antibodies

Product name

H3K27me1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Concentration

0.83 mg/mL

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Dot Blot of Rabbit Histone H3 K27 me1-3 Antibody. Lane 1: Unmodified. Lane 2: me1. Lane 3: me2. Lane 4: me3. Lane 5: ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [1-3 methyl, Lys27, ac] antibody at 0.5µg/ml for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Dot Blot of Rabbit Histone H3 K27 me1-3 Antibody. Lane 1: Unmodified. Lane 2: me1. Lane 3: me2. Lane 4: me3. Lane 5: ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [1-3 methyl, Lys27, ac] antibody at 0.5µg/ml for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.