433270

antibody from Invitrogen Antibodies

Targeting: PANX3 Px3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

433270

Provider

Invitrogen Antibodies

Product name

Pannexin 3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.25 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

References

Submitted references

Human stem cells express pannexins.

Hainz N, Beckmann A, Schubert M, Haase A, Martin U, Tschernig T, Meier C
BMC research notes 2018 Jan 22;11(1):54

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Influence of the three-dimensional culture of human bone marrow mesenchymal stromal cells within a macroporous polysaccharides scaffold on Pannexin 1 and Pannexin 3.

Guerrero J, Oliveira H, Aid R, Bareille R, Siadous R, Letourneur D, Mao Y, Kohn J, Amédée J
Journal of tissue engineering and regenerative medicine 2018 Apr;12(4):e1936-e1949

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Pannexin1 channels act downstream of P2X 7 receptors in ATP-induced murine T-cell death.

Shoji KF, Sáez PJ, Harcha PA, Aguila HL, Sáez JC
Channels (Austin, Tex.) 2014;8(2):142-56

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Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens.

Shahidullah M, Mandal A, Beimgraben C, Delamere NA
Journal of cellular physiology 2012 Apr;227(4):1428-37

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of non-transfected C6 cells (lane B) or cells transfected with rat Pannexin 3 cDNA using Rabbit anti-Pannexin 3 antibody (Product # 433270).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of non-transfected C6 cells (lane B) or cells transfected with rat Pannexin 3 cDNA using Rabbit anti-Pannexin 3 antibody (Product # 433270).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of non-transfected HeLa cells (panel A) and cells transfected with rat Pannexin 3 cDNA using Rabbit anti-Pannexin 3 antibody (Product # 433270).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1 Immunolabeling for Panx1, Panx3, and Cx43 in HBMSCs in 2D or 3D cultures. (a-d) Panx1 immunolabeling at Day 1 and Day 4 in 2D and 3D cultures. (e-h) Panx3 immunolabeling at Days 1 and 4 in 2D and 3D cultures. (i-l) Cx43 immunolabeling at Days 1 and 4 in 2D and 3D cultures. In all cases, the primary antibody was detected with Alexa 488-conjugated IgG (in green) and nuclei stained by DAPI (blue)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

6 Immunohistochemistry analysis of Pannexin1 and 3 in implanted matrices seeded with HBMSCs subcutaneously. Representative area of Panx1 (a-f) and Panx3 (g-l) immunohistochemistry of the transversal section of the tissue-engineering scaffolds after 3 and 8 weeks of implantation. Representative images of low magnification of the transversal sections (a,d,g,j) and higher magnifications of the periphery (Pe; b,e,h,k) and of the centre (Ce; c,f,i,l) of the implants. Representative histological images of Masson's trichrome (MT) staining (m,n,o) of subcutaneously implanted matrices loaded with HBMSCs, after 8 weeks of implantation, with a higher magnification at the periphery (n) and of the centre (o) of the implants