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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- ABIN179727 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN179727, RRID:AB_10956427
- Product name
- anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 19 (CEACAM19) antibody
- Antibody type
- Monoclonal
- Antigen
- genetic immunisation with cDNA encoding human CEACAM19
- Description
- Protein G
- Reactivity
- Human
- Isotype
- IgG
- Antibody clone number
- HY-8H10
- Vial size
- 100 μg
- Concentration
- 2 mg/mL
- Storage
- short term: 2°C - 8°C, long term: -20°C
- Handling
- Avoid repeated freezing and thawing.
Submitted references Carcinoembryonic antigen-related cell adhesion molecules as surrogate markers for EGFR inhibitor sensitivity in human lung adenocarcinoma.
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues.
Kobayashi M, Miki Y, Ebina M, Abe K, Mori K, Narumi S, Suzuki T, Sato I, Maemondo M, Endo C, Inoue A, Kumamoto H, Kondo T, Yamada-Okabe H, Nukiwa T, Sasano H
British journal of cancer 2012 Nov 6;107(10):1745-53
British journal of cancer 2012 Nov 6;107(10):1745-53
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues.
Hammarström S
Seminars in cancer biology 1999 Apr;9(2):67-81
Seminars in cancer biology 1999 Apr;9(2):67-81
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Spectral Confocal Microscopy of CHO cells using HY-8H10. CHO cells were transiently transfected with an expression vector encoding CEACAM19. Binding of HY-8H10 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using HY-8H10. BOSC23 cells were transiently trans-fected with an expression vector encoding either CEACAM19 (red curve) or an irrelevant protein (control transfectant). Binding of HY-8H10 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM19 transfected cells.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified HY-8H10 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM19 transfected cells (red curve).
