Explore
Validate
Learn
ELISA
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ALX-805-089-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-805-089-C100, RRID:AB_11178266
- Product name
- CEACAM19 (human) monoclonal antibody (HY-8H10)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Protein G-affinity purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HY-8H10
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Specificity testing by flow cytometry. Method: FACS analysis of BOSC23 cells using CEACAM19 (human), mAb (HY-8H10) (Prod. No. ALX-805-089). BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM19 (red curve) or an irrelevant protein (control transfectant). Binding of CEACAM19 (human), mAb (HY-8H10) was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM19 transfected cells.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Antibody cross-reactivity with members of the CEA family. Method: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, CEACAM19 (human), mAb (HY-8H10) (Prod. No. ALX-805-089) was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM19 transfected cells (red curve).
