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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- MA5-45234 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AHA1 Monoclonal Antibody (25F2.D9), FITC
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 25F2.D9
- Vial size
- 100 µg
- Concentration
- 1.2 mg/mL
- Storage
- 4° C, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Human Cell lysates showing detection of Aha1 protein. Load: 15 µg. Blocking: 1.5% BSA for 30 minutes at RT. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45234) at 1:1,000 for 2 hours at RT, followed by Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45234) at 1:1,000, followed by FITC Goat Anti-Rat (green).
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45234) at 1:1,000, followed by FITC Goat Anti-Rat (green).
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45234) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45234) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis using HeLa cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45234) at 1:1,000.
- Conjugate
- Green dye
