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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- LS-C395707 - Provider product page
- Provider
- LSBio
- Product name
- FNTB Antibody LS-C395707
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All lanes: FNTB antibody at 6µg/ml Lane 1: Hela whole cell lysate Lane 2: CEM whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: U87 whole cell lysate Lane 6: A375 whole cell lysate Lane 7: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 44 kDa Observed band size: 49 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating FNTB in HepG2 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of FNTB Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: FNTB Antibody (8µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
