Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4916 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal SEZ6L2/BSRP-A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse SEZ6L2/BSRP-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) BSRP-B and rmBSRP-C is observed.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain of FAM19A5-LacZ Knock-in Mice.
Shahapal A, Cho EB, Yong HJ, Jeong I, Kwak H, Lee JK, Kim W, Kim B, Park HC, Lee WS, Kim H, Hwang JI, Seong JY
Frontiers in neuroscience 2019;13:917
Frontiers in neuroscience 2019;13:917
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- SEZ6L2/BSRP-A in Mouse Embryo. SEZ6L2/BSRP-A was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse SEZ6L2/BSRP-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4916) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain (medulla oblongata), spinal cord, trigeminal ganglia, and retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.