RQ7746

antibody from NSJ Bioreagents

Targeting: SYNE3 C14orf139, C14orf49, FLJ25605, LINC00341, NCRNA00341, Nesp3, Nesprin-3, NET53

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7746

Provider

NSJ Bioreagents

Product name

Nesprin 3 Antibody / SYNE3

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Nesprin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) 293T and 2) HeLa cell lysate with Nesprin 3 antibody. Predicted molecular weight ~112 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with Nesprin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human mouse liver tissue with Nesprin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human spleen rupture tissue with Nesprin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human thyroid papillary carcinoma tissue with Nesprin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-87 MG cells with Nesprin 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nesprin 3 antibody.