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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00004534-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004534-M01, RRID:AB_2147699
- Product name
- Mouse Monoclonal Myotubularin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MTM1 - myotubularin 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Novel findings associated with MTM1 suggest a higher number of female symptomatic carriers.
Myopathy in a woman and her daughter associated with a novel splice site MTM1 mutation.
Savarese M, Musumeci O, Giugliano T, Rubegni A, Fiorillo C, Fattori F, Torella A, Battini R, Rodolico C, Pugliese A, Piluso G, Maggi L, D'Amico A, Bruno C, Bertini E, Santorelli FM, Mora M, Toscano A, Minetti C, Nigro V
Neuromuscular disorders : NMD 2016 Apr-May;26(4-5):292-9
Neuromuscular disorders : NMD 2016 Apr-May;26(4-5):292-9
Myopathy in a woman and her daughter associated with a novel splice site MTM1 mutation.
Hedberg C, Lindberg C, Máthé G, Moslemi AR, Oldfors A
Neuromuscular disorders : NMD 2012 Mar;22(3):244-51
Neuromuscular disorders : NMD 2012 Mar;22(3):244-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of MTM1 over-expressed 293 cell line, cotransfected with MTM1 Validated Chimera RNAi ( Cat # H00004534-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MTM1 monoclonal antibody (M01), clone 1C10 (Cat # H00004534-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of MTM1 expression in transfected 293T cell line by MTM1 monoclonal antibody (M01), clone 1C10.Lane 1: MTM1 transfected lysate(69.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Myotubularin Antibody (1C10) [H00004534-M01] - Detection limit for recombinant GST tagged MTM1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of monoclonal antibody to MTM1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
