H00025831-M03

antibody from Novus Biologicals

Targeting: HECTD1 KIAA1131

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

H00025831-M03

Provider

Novus Biologicals

Proper citation

Novus Cat#H00025831-M03, RRID:AB_2117391

Product name

Mouse Monoclonal HECTD1 Antibody

Antibody type

Monoclonal

Description

IgG purified. HECTD1 - HECT domain containing 1

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

The ubiquitin ligase HECTD1 promotes retinoic acid signaling required for development of the aortic arch.

Sugrue KF, Sarkar AA, Leatherbury L, Zohn IE
Disease models & mechanisms 2019 Jan 11;12(1)

---

HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures.

Shen X, Jia Z, D'Alonzo D, Wang X, Bruder E, Emch FH, De Geyter C, Zhang H
Cell communication and signaling : CCS 2017 Jan 5;15(1):2

---

Abnormal labyrinthine zone in the Hectd1-null placenta.

Sarkar AA, Sabatino JA, Sugrue KF, Zohn IE
Placenta 2016 Feb;38:16-23

---

Hectd1 is required for development of the junctional zone of the placenta.

Sarkar AA, Nuwayhid SJ, Maynard T, Ghandchi F, Hill JT, Lamantia AS, Zohn IE
Developmental biology 2014 Aug 15;392(2):368-80

---

Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme.

Sarkar AA, Zohn IE
The Journal of cell biology 2012 Mar 19;196(6):789-800

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: HECTD1 Antibody (1E10) [H00025831-M03] - Detection limit for recombinant GST tagged HECTD1 is approximately 0.3ng/ml as a capture antibody.