PA5-112445

antibody from Invitrogen Antibodies

Targeting: H1-5 H1b, H1F5, H1s-3, HIST1H1B

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112445

Provider

Invitrogen Antibodies

Product name

Phospho-Histone H1.5 (Ser17) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.05 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Phospho Histone H1.5 (Ser17) in MCF-7 whole cell lysate using a Phospho Histone H1.5 (Ser17) polyclonal antibody (Product # PA5-112445) at a concentration of 1 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of Phospho Histone H1.5 (Ser17) in HepG2 cells using a Polyclonal antibody (Product # PA5-112445) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of Phospho Histone H1.5 (Ser17) in HepG2 cells using a Polyclonal antibody (Product # PA5-112445) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.