Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-62230 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMBS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EFSAIILATA GLQRMGWHNR VGQILHPEEC MYAVGQGALG VEVRAKDQDI LDLVGVLHDP ETLLRCIAER AFLRHLEGGC SVPVAVHTAM KDGQLYLTGG VWSLDGSDSI QETMQATIHV PAQHEDGPED DPQLV Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 94%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMBS in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using a HMBS Polyclonal Antibody (Product # PA5-62230).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HMBS in human cell line U-2 OS shows positivity in vesicles. Samples were probed using a HMBS Polyclonal Antibody (Product # PA5-62230).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HMBS in human cell line A-431 using a HMBS Polyclonal Antibody (Product # PA5-62230) shows localization to lipid droplets.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HMBS in human bone marrow shows strong positivity in subsets of hematopoietic cells. Samples were probed using a HMBS Polyclonal Antibody (Product # PA5-62230).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HMBS in human bone marrow and cerebral cortex tissues using HMBS Polyclonal Antibody (Product # PA5-62230). Corresponding HMBS RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HMBS in human cerebral cortex using HMBS Polyclonal Antibody (Product # PA5-62230) shows low expression as expected.